Gene Expression and Regulation
In a bacterial operon system, how can an increase in the concentration of a metabolic end product lead to decreased production of the enzymes responsible for its synthesis?
The metabolic product interferes directly with ribosome function during translation of these enzymes.
The end product acts as a corepressor that activates a repressor protein which binds to the operator site and blocks RNA polymerase.
The surplus metabolite enhances allosteric activation of transcription factors for unrelated genes' promoters.
The end product increases degradation rate of mRNA coding for these enzymes through ribonuclease activity.
Which molecule is responsible for carrying the genetic code from DNA to the ribosome for protein synthesis?
tRNA
DNA polymerase
rRNA
mRNA
What is an activator in positive gene regulation?
A protein that turns on RNA polymerase
Activators are not present in positive gene regulation
A protein that induces repressors to make them inactive so transcription occurs
A protein that binds upstream of the DNA stimulates the transcription of a gene
What role do regulatory sequences play in gene expression?
They lead to heritable changes in gene function without changes to DNA sequence.
They directly form the structure of chromosomes.
They control the activation or repression of genes.
They encode for specific proteins.
How does a decrease in light intensity most likely affect photosynthetic organisms over time?
Greater utilization of stored fats and proteins for cellular energy needs.
Increased reliance on anaerobic pathways like lactic acid fermentation.
Reduced energy capture for synthesizing carbohydrates via photosynthesis.
Elevated rates of photorespiration resulting in more efficient growth.
When examining a pedigree chart for a particular trait, what indicates that the trait is likely autosomal recessive?
Only males are affected by the trait.
Affected individuals are usually homozygous dominant.
The trait appears in every generation.
Most affected individuals have unaffected parents.
In Lac Operon Model, What Is The Result If Lactose Is Present But Glucose Levels Are High?
The Repressor Protein Stays Bound To The Operator
RNA Polymerase Cannot Bind To The Promoter
Allolactose Does Not Act As An Inducer
The Lac Operon Will Not Be Fully Activated

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When considering post-transcriptional regulation, what role do microRNAs (miRNAs) play when they are complementary to sequences on mRNA molecules?
They increase efficiency during translation by acting as adaptors between codons on mRNAs and amino acids carried by tRNAs.
They facilitate faster mRNA processing by aiding in the addition of poly(A) tails and caps needed for stability and exportation from nucleus.
They can lead to mRNA degradation or block translation by binding mRNA and recruiting other proteins that interfere with these processes.
They enhance ribosomal attachment to mRNAs through alterations in secondary structures making them more accessible for translation initiation factors.
How do microRNAs (miRNAs) silence gene expression at the post-transcriptional level?
They bind complementary sequences on mRNA molecules leading to degradation or repression translation
...
They catalyze addition methyl groups onto DNA suppressing transcriptional activity
They facilitate acetylation histones which results tighter packing preventing access RNA polymerase
How do corepressors contribute to gene regulation?
Corepressors enhance production from transcription
Corepressors bind themselves to the promotor and halt transcription
Corepressors cooperate with repressors to switch an operon off
Corepressors terminate the transcription process