All Flashcards
What are the key differences between competitive and noncompetitive inhibitors?
Competitive: Binds to active site, blocks substrate. Noncompetitive: Binds elsewhere, changes enzyme shape.
What is the effect of increasing substrate concentration on enzyme activity (up to saturation)?
Increases the reaction rate up to a saturation point.
What is the effect of extreme temperature or pH on enzyme activity?
Can lead to denaturation, causing the enzyme to lose its shape and function.
What happens when a competitive inhibitor is added to an enzyme reaction?
The reaction rate decreases because the inhibitor blocks the active site.
What happens when a noncompetitive inhibitor binds to an enzyme?
The enzyme's shape changes, reducing its activity.
What are the steps of enzyme catalysis?
- Enzyme and substrate bind, forming an enzyme-substrate complex. 2. Enzyme lowers the activation energy. 3. Reaction proceeds, forming product. 4. Product is released, and the enzyme is free to catalyze another reaction.