Intermolecular Forces and Properties
Which phase in chromatography stays stationary during the process?
The mobile phase.
The gas phase if present in the system.
The solid phase.
The liquid phase no matter where it is located.
What is the purpose of using a mobile phase in chromatography?
To act as a stationary phase
To increase the temperature for separation
To transport the sample through the stationary phase
To permanently bind with the sample
Considering principles behind ion-exchange chromatography, how might altering pH buffer solution impact retention times differently charged analyte ions?
Altering has no significant effect since charge density resin sites remains constant over wide range conditions thereby maintaining consistent affinity levels among diverse ion populations encountered during analysis.
Changing pH could either shorten lengthen depending whether adjusted towards away analyte's pKa resulting enhanced or diminished interaction resin function groups respectively.
Adjustments invariably compel all types sampled entities behave identically respect flow dynamics encompassed method application neglecting consider variation influences exerted distinct molecular structures bear upon overall system efficiency utility.
Altered leads indiscriminate increase general attributed solely heightened electrical conductivity introduced via addition excess H+ OH– ions found altered buffers used modifying running parameters accordingly.
If a liquid substance with hydrogen bonding is subjected to thin-layer chromatography, how would its Rf value likely compare to that of a similar molecular weight substance with only London dispersion forces?
It would have an identical Rf value as molecular weight solely determines this.
It would have a lower Rf value because of stronger intermolecular forces.
It would have a higher Rf value due to increased vapor pressure.
It would have a varying Rf value not predictable by intermolecular forces.
Which type of intermolecular force must be overcome to separate the components in chromatography?
Metallic bonds
Covalent bonds
Van der Waals forces
Ionic bonds
Which of the following errors would NOT cause a spectrophotometer to produce incorrect readings?
Switching the standard cuvette concentrations while setting up the spectrophotometer
Leaving fingerprints on a cuvette
Inserting a cuvette sideways
Recording the concentration of the sample as 10 times greater than its actual value
Which interaction is primarily responsible for the retention of a compound on a polar stationary phase in chromatography?
Hydrogen bonding
Ionic bonding
Covalent bonding
London dispersion forces

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When analyzing an unknown compound via reverse-phase HPLC which characteristic property most strongly influences its retention time?
Hydrophobicity/hydrophilicity balance within compound structure.
Presence absence chemical functional groups
The total number carbon atoms present inside molecular structure
molecular symmetry asymmetry
Why must samples be spotted above water level in paper chromatography?
They would evaporate
They would stain into glassware if spotted below water level
They would release toxic fumes at lower levels
Because they would dissolve into water if submerged
What outcome is expected when performing paper chromatography using water as a solvent on a mixture containing both hydrophilic and lipophilic substances?
Lipophilic substances will travel further since they are less attracted to the water-based mobile phase than hydrophilic substances are.
No differentiation occurs because paper chromatography cannot distinguish between different categories of molecular polarity effectively.
Hydrophilic substances will travel further up the paper than lipophilic substances due to stronger solvent-substrate interactions.
Both types of substances remain at baseline because water only functions effectively as a universal solvent with salts and sugars primarily.